Cryo electron microscopy and structure-based mutagenesis reveal that the bacteriophage BPP-1 contains two of the three major recognized viral folds, one of which exhibits a new topology.
Development from dual-origin mesenchyme with common competence explains anatomical parallels and shared patterning of the jawed vertebrate gill arch and paired fin skeletons.
Human vitamin K epoxide reductase (VKOR) has a four transmembrane domain topology that supports the use of a homology model, enabling identification of active site residues and human variant impact.
Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
The ER membrane protein complex (EMC) facilitates the correct topology of the flavivirus non-structural proteins NS4A and NS4B at the ER membrane critical for viral replication.
Coarse-grained modeling reveals a new mechanism for multispanning membrane protein topogenesis, in which misintegrated configurations of the proteins undergo post-translational annealing to reach final, fully integrated multispanning topologies.