Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.

Cryo electron microscopy and structure-based mutagenesis reveal that the bacteriophage BPP-1 contains two of the three major recognized viral folds, one of which exhibits a new topology.

The ancestor of all known jawed vertebrates possessed a maxilla and premaxilla and was similar in morphology to the 'maxillate placoderms' from China.

Development from dual-origin mesenchyme with common competence explains anatomical parallels and shared patterning of the jawed vertebrate gill arch and paired fin skeletons.

Human vitamin K epoxide reductase (VKOR) has a four transmembrane domain topology that supports the use of a homology model, enabling identification of active site residues and human variant impact.

Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.

Precise genome editing by homology-directed repair occurs more efficiently with a chromatin donor template than with a naked DNA donor template.

The ER membrane protein complex (EMC) facilitates the correct topology of the flavivirus non-structural proteins NS4A and NS4B at the ER membrane critical for viral replication.

Coarse-grained modeling reveals a new mechanism for multispanning membrane protein topogenesis, in which misintegrated configurations of the proteins undergo post-translational annealing to reach final, fully integrated multispanning topologies.

The planarian mex3-1 gene is required for non-stem cell fates and suppresses a stem cell fate in differentiating cells.