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    1. Computational and Systems Biology
    2. Developmental Biology

    Temporal modulation of collective cell behavior controls vascular network topology

    Esther Kur et al.
    Tissue-specific temporal regulators impinge on the VEGF/Delta-Notch pathway to vary tip cell selection pace yielding diverse densities of vascular network.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology

    Sumit Pawar et al.
    Efficient targeting of membrane proteins from the endoplasmic reticulum (ER) to the inner nuclear membrane depends on GTP hydrolysis by Atlastin GTPases and their function in maintaining an interconnected topology of the ER network.
    1. Structural Biology and Molecular Biophysics

    The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses

    Xabier Agirrezabala et al.
    The high-resolution structure of a filamentous flexible plant virus shows that there is structural homology between its coat protein and the nucleoproteins of an unrelated group of enveloped RNA animal viruses.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

    Steven Lin et al.
    Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
    1. Genetics and Genomics

    Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

    Melissa A Chiasson et al.
    Human vitamin K epoxide reductase (VKOR) has a four transmembrane domain topology that supports the use of a homology model, enabling identification of active site residues and human variant impact.
    1. Genetics and Genomics

    An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms

    Oguz Kanca et al.
    Optimization of homology donor DNA production and integration schemes facilitates homologous recombination in vivo and in cell culture in Drosophila melanogaster..
    1. Cell Biology

    IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact

    Benjamin Bonneau et al.
    IRBIT and the Bcl-2 homolog Bcl2l10 form a complex at the endoplasmic reticulum that controls Ca2+ signaling, however IRBIT turns into an apoptosis facilitator following stress and inhibits anti-apoptotic activity of Bcl2l10.
    1. Structural Biology and Molecular Biophysics

    Binding and transport of D-aspartate by the glutamate transporter homolog GltTk

    Valentina Arkhipova et al.
    Glutamate transporter homologs can efficiently bind and transport both L- and D- enantiomeric substrates with only slight rearrangements in the binding site.
    1. Developmental Biology
    2. Genetics and Genomics

    Efficient targeted integration directed by short homology in zebrafish and mammalian cells

    Wesley A Wierson et al.
    Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.