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    1. Microbiology and Infectious Disease

    Predictors of SIV recrudescence following antiretroviral treatment interruption

    Mykola Pinkevych, Christine M Fennessey ... Miles P Davenport
    The frequency of SIV reactivation from latency after treatment interruption is not directly related to measures of SIV DNA, RNA, immune activation or immune response in peripheral blood.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Stress conditions promote Leishmania hybridization in vitro marked by expression of the ancestral gamete fusogen HAP2 as revealed by single-cell RNA-seq

    Isabelle Louradour, Tiago Rodrigues Ferreira ... David Sacks
    Following exposure of Leishmania culture promastigotes to DNA stress, single-cell RNA sequencing reveals discrete clusters of cells associated with efficient generation of hybrids between different species and strains in vitro marked by expression of the ancestral gamete fusogen HAP2.
    1. Epidemiology and Global Health

    The Anopheles gambiae 2La chromosome inversion is associated with susceptibility to Plasmodium falciparum in Africa

    Michelle M Riehle, Tullu Bukhari ... Kenneth D Vernick
    A common chromosome inversion in African malaria mosquito (Anopheles gambiae) is associated with differences in malaria infection, adult resting behavior and ecology, and may aid the most efficient vectors to evade malaria control.
    1. Developmental Biology

    Response to Nodal morphogen gradient is determined by the kinetics of target gene induction

    Julien Dubrulle, Benjamin M Jordan ... Alexander F Schier
    A kinetic model of morphogen interpretation is more suitable than classic threshold or ratchet models to understand how a signal gradient generates different target gene expression patterns.
    1. Microbiology and Infectious Disease

    Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae

    Noémie Matthey, Sandrine Stutzmann ... Melanie Blokesch
    Whole-genome sequencing reveals the remarkable extent of horizontally moving genetic material in naturally competent Vibrio cholerae after a prey-killing DNA acquisition process.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Genome-wide identification of lineage and locus specific variation associated with pneumococcal carriage duration

    John A Lees, Nicholas J Croucher ... Stephen D Bentley
    Sequence changes in the pneumococcal genome explain most of the variability in duration of asymptomatic carriage with serotype, antibiotic resistance and prophage accounting for the largest effects.
    1. Cell Biology

    SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation

    David W Sanders, Chanelle C Jumper ... Clifford P Brangwynne
    A high-throughput microscopy screen for drugs that modulate SARS-CoV-2 spike-mediated membrane fusion identifies an essential role for cholesterol in both virus entry and syncytia formation.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Multiple serine transposase dimers assemble the transposon-end synaptic complex during IS607-family transposition

    Wenyang Chen, Sridhar Mandali ... Reid C Johnson
    Genetic, structural, and biochemical analyses of IS607-family transposons shows that the DNA translocation reaction proceeds very differently from other reactions promoted by serine recombinases.
    1. Developmental Biology
    2. Microbiology and Infectious Disease

    Region-specific regulation of stem cell-driven regeneration in tapeworms

    Tania Rozario, Edward B Quinn ... Phillip A Newmark
    Tapeworms grow and regenerate using stem cells distributed throughout their bodies, but their regeneration competence relies upon the unique micro-environment created by the head and neck.
    1. Computational and Systems Biology
    2. Developmental Biology

    The pattern of nodal morphogen signaling is shaped by co-receptor expression

    Nathan D Lord, Adam N Carte ... Alexander F Schier
    Ligand capture by cell surface receptor complexes sets the shape of the Nodal signaling pattern in zebrafish.