An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.
Promoter interactome maps in human embryonic stem cells (ESCs) and ESC-derived early neuroectodermal progenitors link distal enhancers to putative target genes, reveal lineage-specific cis-regulatory architecture and shed light on the logic of gene regulation by multiple enhancers.
The smoking cessation drug varenicline (Chantix) and other weak base ligands reduce a4b2-type nicotinic receptor (a4b2R) upregulation through slow intracellular release due to selective trapping in acidic vesicles that contain a4b2Rs.
Improved characterisation of human embryonic lung development highlights human-mouse differences and facilitates the development of defined culture conditions for the expansion of self-renewing, multipotent human lung epithelial progenitor cells.
A combination of transcriptomics, proteomics and modelling identifies a network of interacting protein phosphatases that act as a biological switch to move cells from the stem cell compartment to the differentiated compartment in cultured human epidermis.