Acting in neuronal stem cells, temporal transcription factors, as a class of molecules, are uniquely potent determinants of circuit membership that establish expected patterns of wiring in the motor system.
The effective higher-order binding cooperativities arising from an ensemble of dynamically interchanging conformations are identified and shown to fully describe integration of binding information at thermodynamic equilibrium.
Propagation, speed and shapes of genetic waves of expression during development can be modeled by a simple interplay between two transcriptional modules (dynamic/static), which explains robustness and precision of patterning.
Live quantitative monitoring of transcriptional bursting reveals that enhancers responding to different regulators use the same kinetic strategy to produce a complex composite pattern of developmental expression.
Proper development depends on establishing precise gene expression patterns in spite of the inherent noise in transcription, shadow enhancers buffer this noise by binding distinct input transcription factors.
The patterns of chromatin architecture that underlie the initial embryonic cell fate decisions are established during a period of intense cell cycle activity, and these patterns are stably maintained even in highly condensed mitotic chromatin.