359 results found
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism of thermosensory function of human heat shock transcription factor Hsf1

    Nikolai Hentze et al.
    Heat-induced local unfolding allows Hsf1 to form trimers and bind to DNA, which depends on Hsf1 concentration and is promoted, not inhibited, by Hsp90.
    1. Structural Biology and Molecular Biophysics

    HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon

    Zainab Ahdash et al.
    Hydrogen-deuterium exchange mass spectrometry reveals nucleotide-driven conformational regulation of Sec protein-channel to help impose directionality for protein transport through the Sec complex.
    1. Structural Biology and Molecular Biophysics

    Auto-regulation of Rab5 GEF activity in Rabex5 by allosteric structural changes, catalytic core dynamics and ubiquitin binding

    Janelle Lauer et al.
    Understanding the complex auto-regulatory mechanisms for guanine nucleotide exchange factors is critically important for fully appreciating the layers of control for small GTPases.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Tracing a protein’s folding pathway over evolutionary time using ancestral sequence reconstruction and hydrogen exchange

    Shion An Lim et al.
    Pulsed-labeling hydrogen exchange on the ribonuclease H family show that the major folding intermediate is conserved over three billion years of evolution, but the path leading to this intermediate varies.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Ric-8A, a G protein chaperone with nucleotide exchange activity induces long-range secondary structure changes in Gα

    Ravi Kant et al.
    Hydrogen-Deuterium exchange experiments show that Ric-8A induces similar dynamic changes in the structure of Gα as G protein-coupled receptors, yet protects a larger surface of the nucleotide-binding Ras domain.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes

    Yohei Ohashi et al.
    The lipid kinase VPS34 complexes I and II are both activated by unsaturation of substrate and non-substrate lipids, curvature, electrostatics and polyphosphoinositides, which play roles in localisation and cellular function.
    1. Cell Biology

    Unstructured regions in IRE1α specify BiP-mediated destabilisation of the luminal domain dimer and repression of the UPR

    Niko Amin-Wetzel et al.
    Client protein-driven reversal of endoplasmic reticulum chaperone (BiP) mediated-repression is revealed as a principal component of the regulation of the unfolded protein response transducer IRE1 in cells.
    1. Chromosomes and Gene Expression

    Crl activates transcription by stabilizing active conformation of the master stress transcription initiation factor

    Juncao Xu et al.
    The cryo-EM structure of Escherichia coli Crl–TAC reveals the mechanism of DNA-contact-independent transcription activation.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    The Cac1 subunit of histone chaperone CAF-1 organizes CAF-1-H3/H4 architecture and tetramerizes histones

    Wallace H Liu et al.
    Biophysical and biochemical approaches reveal new insights into the architecture of CAF-1 and the unique mechanism by which CAF-1 tetramerizes histones H3/H4.
    1. Structural Biology and Molecular Biophysics

    Interplay of disordered and ordered regions of a human small heat shock protein yields an ensemble of ‘quasi-ordered’ states

    Amanda F Clouser et al.
    Multiple iso-energetic-specific interactions involving the intrinsically-disordered region of sHSP HSPB1 define a quasi-ordered state, providing insights into inherited disease-associated mutations within the region that are thought to be disordered.

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