Implementation and characterisation of plasma-focused ion beams for volumetric imaging of cryogenically preserved cells and tissues for ultrastructural examination in their native state.
Marta Pallotto, Paul V Watkins ... Kevin L Briggman
Automated segmentation of neurons and identification of synapses in electron micrographs is significantly improved by using simple modifications to chemical fixation protocols that preserve extracellular space in the brain.
Martial Sankar, Kaisa Nieminen ... Christian S Hardtke
Combining high-resolution imaging with automated image segmentation and supervised machine learning achieves accurate cellular feature extraction and automated cell type recognition in a large-scale developmental process.
MotionTracking is freely available software that can generate multi-scale geometrical models of mammalian tissue and perform quantitative analysis of tissue architecture.
The DIAMonDS can automatically and sequentially identify time points of multiple life cycle events such as pupariation, eclosion, and death in individual flies at high temporal resolution and large scale.