Cyanine fluorophores are encoded as non-canonical amino acids to produce functional proteins in cell-free translation systems and live cells for single-molecule imaging.
Aging is a process characterized by gradual metabolome remodeling, deceleration of the remodeling in late life and under conditions that extend lifespan, and a mortality-associated pattern of cumulative damage.
Establishment of two-photon imaging with a 1100-nm laser, which underfills the objective's back aperture, detects activity of multiple neurons in the prelimbic area and hippocampal CA1 region of the intact mouse brain.
A new platform for imaging live Drosophila adults yields vivid movies of the midgut over prolonged time scales, opening the door to the real-time study of organ renewal dynamics in a near-native context.
A luminescence-based imaging system is presented that allows soil-based environmental stimuli to be studied in root systems under physiologically relevant conditions.
Epigenetic drift of H3K27me3 is one of the molecular mechanisms that contribute to aging, and stimulation of glycolysis promotes metabolic health and longevity.
Using noise suppression, real-time imaging of glucose metabolism is possible by 13C MRI and reveals previously undetected metabolic differences within tumors.
Iodixanol supplementation provides a simple method for tuning the refractive index of live imaging media, which can greatly improve resolution and penetration depth in live imaging experiments.
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
