Nicolas P Andrews, Justin X Boeckman ... James S Trimmer
This paper describes an effective pipeline for conversion of conventional monoclonal antibodies into recombinant form that results in an IgG subclass switch that greatly expands their utility for multiplex labeling.
The augmentation of resin-embedded sample blocks with magnetic resin allows remote actuation of ultrathin sections and deposition at high packing density onto silicon wafers for correlative light and electron microscopy.
The medial entorhinal cortex is important for spatial memory formation and matures from dorsal, where smaller spatial-scales are represented, to ventral, where larger spatial-scales are represented.
Tomas Mediavilla, Özgün Özalay ... Daniel J Marcellino
Learning produces dynamic white matter volumetric changes in the rodent brain observed by voxel-based morphometry on longitudinal structural MRI for which estimated white matter volume reflects adaptive myelination in the cortex.
Timothy Erickson, Clive P Morgan ... Teresa Nicolson
A zebrafish model for a particular form of human deafness (DFNB63) changes our view of this disease by revealing a defect in the localization of Transmembrane channel-like proteins that are essential for mechanotransduction in sensory cells.
Inhibitory cerebellar feedback to the inferior olive is central to computational models of learning, but novel targets of these neurons identified here expand their potential roles.
Astrocytes decode temporal interneuron activity and transform inhibitory into excitatory signals that impact excitatory synaptic strength and neuronal circuit function.
Andrew E Worthy, Joanna T Anderson ... Francisco J Alvarez
Major differences among the main spinal V1 inhibitory interneuron subgroups were revealed based on neurogenesis, circuit placement, and motoneuron targeting, and Foxp2-V1 interneurons were identified tightly coupled to limb control.
STIM1 regulates the contractility of blood vessels and blood pressure by organizing membrane contact sites and ion channel activity in vascular smooth muscle cells in a manner that is independent of its canonical function in store-operated Ca2+entry.
John H Day, Catherine M Della Santina ... Laurie A Boyer
A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.