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    1. Neuroscience

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain

    Nicolas P Andrews, Justin X Boeckman ... James S Trimmer
    This paper describes an effective pipeline for conversion of conventional monoclonal antibodies into recombinant form that results in an IgG subclass switch that greatly expands their utility for multiplex labeling.
    1. Neuroscience

    MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

    Thomas Templier
    The augmentation of resin-embedded sample blocks with magnetic resin allows remote actuation of ultrathin sections and deposition at high packing density onto silicon wafers for correlative light and electron microscopy.
    1. Neuroscience

    Structural development and dorsoventral maturation of the medial entorhinal cortex

    Saikat Ray, Michael Brecht
    The medial entorhinal cortex is important for spatial memory formation and matures from dorsal, where smaller spatial-scales are represented, to ventral, where larger spatial-scales are represented.
    1. Neuroscience

    Learning-related contraction of gray matter in rodent sensorimotor cortex is associated with adaptive myelination

    Tomas Mediavilla, Özgün Özalay ... Daniel J Marcellino
    Learning produces dynamic white matter volumetric changes in the rodent brain observed by voxel-based morphometry on longitudinal structural MRI for which estimated white matter volume reflects adaptive myelination in the cortex.
    1. Neuroscience

    Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt)

    Timothy Erickson, Clive P Morgan ... Teresa Nicolson
    A zebrafish model for a particular form of human deafness (DFNB63) changes our view of this disease by revealing a defect in the localization of Transmembrane channel-like proteins that are essential for mechanotransduction in sensory cells.
    1. Neuroscience

    Diverse inhibitory projections from the cerebellar interposed nucleus

    Elena N Judd, Samantha M Lewis, Abigail L Person
    Inhibitory cerebellar feedback to the inferior olive is central to computational models of learning, but novel targets of these neurons identified here expand their potential roles.
    1. Neuroscience

    Activity-dependent switch of GABAergic inhibition into glutamatergic excitation in astrocyte-neuron networks

    Gertrudis Perea, Ricardo Gómez ... Alfonso Araque
    Astrocytes decode temporal interneuron activity and transform inhibitory into excitatory signals that impact excitatory synaptic strength and neuronal circuit function.
    1. Neuroscience

    Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity

    Andrew E Worthy, Joanna T Anderson ... Francisco J Alvarez
    Major differences among the main spinal V1 inhibitory interneuron subgroups were revealed based on neurogenesis, circuit placement, and motoneuron targeting, and Foxp2-V1 interneurons were identified tightly coupled to limb control.
    1. Structural Biology and Molecular Biophysics

    STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

    Vivek Krishnan, Sher Ali ... Scott Earley
    STIM1 regulates the contractility of blood vessels and blood pressure by organizing membrane contact sites and ion channel activity in vascular smooth muscle cells in a manner that is independent of its canonical function in store-operated Ca2+entry.
    1. Cell Biology

    High-throughput expansion microscopy enables scalable super-resolution imaging

    John H Day, Catherine M Della Santina ... Laurie A Boyer
    A simple and accessible method for high-throughput super-resolution fluorescence microscopy that is compatible with commonly used staining practices, 96-well cell culture plates, and confocal microscopes.