A new approach uses heat-induced antigen retrieval to enable tissue clearing, multiple-round immunostaining, and expansion microscopy for volumetric imaging of centimeter-scale clinical formalin-fixed paraffin-embedded specimens.

Analyses using His6-HA-SUMO1 and SUMO1 KO mice indicate that SUMO1 modification of synaptic proteins is either absent or cannot be detected in brain and cultured neurons.

This paper describes an effective pipeline for conversion of conventional monoclonal antibodies into recombinant form that results in an IgG subclass switch that greatly expands their utility for multiplex labeling.

Ultrastructural and imaging studies of cochlear ribbon synapses demonstrate cis- and trans-synaptic molecular and structural effects in the absence of the AMPA-type glutamate receptor subunit GluA3 during mouse development in ambient sound levels.

Imaging of energy status and autophagy during neuronal migration revealed that ATP/ADP levels dynamically change during the migratory and stationary phases and that ATP reduction induces autophagy to maintain migration.

Connectomic analysis identifies the complex circuits of a visual motion-sensing neuron that qualify them to generate direction-selective motion sensing signals using both Hassenstein-Reichardt and Barlow-Levick models.

Conditions for multiplexed antibody labeling in mouse brain tissue translates to effective labeling in human brain tissue when samples are prepared similarly.

Different numbers of Munc13-1 molecules build up the specialized regions of the presynaptic plasma membrane called the release sites.

The direct interaction of R-type Ca²⁺ channel Cav2.3 and GABA_B receptor auxiliary subunits in the active zone of medial habenula terminals scales synaptic strength independent of GABA_B receptor activation.

Learning produces dynamic white matter volumetric changes in the rodent brain observed by voxel-based morphometry on longitudinal structural MRI for which estimated white matter volume reflects adaptive myelination in the cortex.