Barcoded rabies virus tracing combined with single-cell RNAseq and in situ sequencing achieves high-throughput retrograde projection mapping and synaptic connectivity mapping at cellular resolution.
Development and application of highly sensitive in situ transcriptomics method, Flura-seq, in identifying dynamic organ-specific transcriptomes in early stage breast cancer metastasis have been described.
CUT&RUN (Cleavage Under Targets & Release Using Nuclease) profiles antibody-targeted DNA-binding proteins in situ with high resolution and low background, providing a simple, robust and scalable alternative to chromatin immunoprecipitation.
A simple modification of CUT&Tag chromatin profiling method provides high-quality chromatin accessibility maps and indicates that 'open' chromatin results from a transcription-coupled process at promoters and enhancers.
In the central amygdala, transcriptomic definition of cell types and corresponding spatial transcriptomic analysis reveals major regional differences in molecular organization and relates newly identified molecularly defined cell types to major axon projection targets.
Dongjin R Lee, Christopher Rhodes ... Timothy J Petros
Single-cell sequencing combined with in situ hybridizations reveal dynamic patterns of differentially expressed genes throughout the ventricular and subventricular zone of the embryonic mouse brain, which likely play important roles in cell fate determination.
A cellular map of a deep-sea mussel gill unveils how distinct cell types collaborate to cultivate intracellular symbiotic bacteria, providing insights into host–symbiont interactions and environmental adaptation in bivalves.
The lncRNA pCharme controls the expression of cardiomyocyte maturation genes and heart development by orchestrating the formation of MATR3-enriched nuclear condensates.