1,556 results found
    1. Neuroscience

    Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing

    Aixin Zhang, Lei Jin ... Xiaoyin Chen
    Barcoded rabies virus tracing combined with single-cell RNAseq and in situ sequencing achieves high-throughput retrograde projection mapping and synaptic connectivity mapping at cellular resolution.
    1. Cancer Biology

    Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization

    Harihar Basnet, Lin Tian ... Joan Massagué
    Development and application of highly sensitive in situ transcriptomics method, Flura-seq, in identifying dynamic organ-specific transcriptomes in early stage breast cancer metastasis have been described.
    1. Chromosomes and Gene Expression

    An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites

    Peter J Skene, Steven Henikoff
    CUT&RUN (Cleavage Under Targets & Release Using Nuclease) profiles antibody-targeted DNA-binding proteins in situ with high resolution and low background, providing a simple, robust and scalable alternative to chromatin immunoprecipitation.
    1. Chromosomes and Gene Expression

    Efficient chromatin accessibility mapping in situ by nucleosome-tethered tagmentation

    Steven Henikoff, Jorja G Henikoff ... Kami Ahmad
    A simple modification of CUT&Tag chromatin profiling method provides high-quality chromatin accessibility maps and indicates that 'open' chromatin results from a transcription-coupled process at promoters and enhancers.
    1. Neuroscience

    Molecular and anatomical characterization of parabrachial neurons and their axonal projections

    Jordan L Pauli, Jane Y Chen ... Richard D Palmiter
    A guide summarizing the diversity of cell types in the parabrachial nucleus.
    1. Neuroscience

    Multimodal mapping of cell types and projections in the central nucleus of the amygdala

    Yuhan Wang, Sabine Krabbe ... Scott M Sternson
    In the central amygdala, transcriptomic definition of cell types and corresponding spatial transcriptomic analysis reveals major regional differences in molecular organization and relates newly identified molecularly defined cell types to major axon projection targets.
    1. Developmental Biology
    2. Neuroscience

    Transcriptional heterogeneity of ventricular zone cells in the ganglionic eminences of the mouse forebrain

    Dongjin R Lee, Christopher Rhodes ... Timothy J Petros
    Single-cell sequencing combined with in situ hybridizations reveal dynamic patterns of differentially expressed genes throughout the ventricular and subventricular zone of the embryonic mouse brain, which likely play important roles in cell fate determination.
    1. Ecology

    Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing

    Hao Wang, Kai He ... Chaolun Li
    A cellular map of a deep-sea mussel gill unveils how distinct cell types collaborate to cultivate intracellular symbiotic bacteria, providing insights into host–symbiont interactions and environmental adaptation in bivalves.
    1. Chromosomes and Gene Expression

    Chromatin Mapping: A cut above

    Chongsheng He, Roberto Bonasio
    A new technique called CUT&RUN can map the distribution of proteins on the genome with higher resolution and accuracy than existing approaches.
    Version of Record
    Insight
    1. Chromosomes and Gene Expression
    2. Developmental Biology

    The long noncoding RNA Charme supervises cardiomyocyte maturation by controlling cell differentiation programs in the developing heart

    Valeria Taliani, Giulia Buonaiuto ... Monica Ballarino
    The lncRNA pCharme controls the expression of cardiomyocyte maturation genes and heart development by orchestrating the formation of MATR3-enriched nuclear condensates.

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