Human yolk sac-like cells, which share characteristics with the post-implantation human hypoblast, can model the interaction between the epiblast and hypoblast that occurs during early human development.
Quantitative FRET UPR induction assay is used to measure IRE1 and BIP association and dissociation by a variety of ER misfolded proteins and by an important BiP substrate-binding domain mutant, significantly enhancing the evidence for the allosteric UPR induction model.
Analysis of neo-splicetope-specific T cell responses strongly questions the idea that in vitro proteasome-catalyzed peptide splicing reaction simulates the in vivo situation with the same high fidelity as the in vitro generation of non-spliced epitopes.
The de novo selection of a mutation responsible for Plasmodium falciparum in vitro artemisinin resistance is confirmed in Guyana, making artemisinin combination therapies vulnerable to complete resistance in this region.
A combination of an in vitro mitochondrial fusion assay with electron cryo-tomography (cryo-ET) reveals distinct steps and protein complexes during mitofusin-dependent fusion between adjacent mitochondrial outer membranes.