409 results found
    1. Structural Biology and Molecular Biophysics

    Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies

    Lars-Anders Carlson et al.
    In vitro reconstitution shows how HIV-1 Gag assemblies on membranes can package the RNA genome in the presence of a vast excess of competing cellular RNAs, and that selectivity and immature lattice assembly are deeply intertwined with one another.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution

    Scott M Coyle, Wendell A Lim
    A systems level reconstitution of H-Ras GTPase signaling reveals a rich space of tunable dynamic outputs and clarifies the consequences of oncogenic perturbations to signaling.
    1. Physics of Living Systems

    Mapping out Min protein patterns in fully confined fluidic chambers

    Yaron Caspi, Cees Dekker
    The geometry selection rules of dynamic Min protein patterns are determined in fully confined fluidic chambers, showing that both oscillations and running waves are derivatives of spiral rotations that are established as the majority pattern.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Design principles of a microtubule polymerase

    Elisabeth A Geyer et al.
    Antagonism between binding to unpolymerized subunits and to the lattice leads to a ratcheting model for the processive action of a microtubule polymerase.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Symmetry breaking in reconstituted actin cortices

    Enas Abu Shah, Kinneret Keren
    A reconstituted system has been developed that self-organizes into dynamic actin cortices capable of spontaneous polarization, similar to the initial cortical polarization observed in cells during embryogenesis and development.
    1. Cell Biology

    RNA-directed activation of cytoplasmic dynein-1 in reconstituted transport RNPs

    Mark A McClintock et al.
    Reconstitution of dynein-based mRNA transport with purified components reveals a pivotal role of an RNA localisation signal in controlling the assembly and activity of the translocation machinery.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Structural insight into TPX2-stimulated microtubule assembly

    Rui Zhang et al.
    A combination of cryo-electron microscopy of TPX2 bound to microtubules and in vitro reconstitution experiments reveals a novel microtubule interaction mode that explains how TPX2 promotes microtubule nucleation and stabilization.
    1. Cell Biology

    Lissencephaly-1 is a context-dependent regulator of the human dynein complex

    Janina Baumbach et al.
    An in vitro reconstitution approach reveals context-dependent roles of Lissencephaly-1 in the regulation of dynein-dynactin behaviour on dynamic microtubules.
    1. Biochemistry and Chemical Biology
    2. Plant Biology

    Identification and reconstitution of the rubber biosynthetic machinery on rubber particles from Hevea brasiliensis

    Satoshi Yamashita et al.
    The first de novo synthesis of polyisoprenoids correspond to natural rubber in vitro with the recombinant proteins is achieved by identification and cell-free translation-coupled reconstitution of the rubber biosynthetic machinery on rubber particles.
    1. Cell Biology

    Reconstitution of dynein transport to the microtubule plus end by kinesin

    Anthony J Roberts et al.
    In vitro reconstitution reveals how a system of molecules works together to transport the motor protein dynein to the start of its track.

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