Gene co-expression analysis identifies coherent transcriptional patterns driven by distinct cell types in the mouse incisor, and functional studies of candidate genes reveal how the tissues are maintained through stem cell-fueled renewal.
Proper development depends on establishing precise gene expression patterns in spite of the inherent noise in transcription, shadow enhancers buffer this noise by binding distinct input transcription factors.
Structure specific nucleases that act in DNA replication, repair and recombination actively mold their DNA while transforming their own structure to achieve precise cleavage of their cognate DNA and avoid the deleterious cleavage of noncognate DNA.
X-ray crystallography reveals that the Dna2 nuclease-helicase contains a long tunnel through which single-stranded DNA threads, and an allosteric mechanism for displacing the DNA-binding protein Rpa that restricts cleavage to the proper polarity.
A mouse model of endometriosis reveals that moderate doses of Δ9-tetrahydrocannabinol produce clear regression of ectopic endometrial growths, pronounced alleviation of pelvic pain and unexpected memory improvements.