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Rapid forward engineering can be accomplished using cell-free systems, as demonstrated by the implementation and characterization of novel genetic oscillators in a cell-free system and their consequent transfer to cells.

The mutation of Lys125 in connexin26 to glutamate, a carbamylation mimetic, pushes the equilibrium of the gap junction channel seen in cryo-EM towards a more closed conformation.

Serial cell-to-cell transfer of lipoprotein toxins by outer membrane exchange provides a potent platform for kin discrimination in Myxococcus xanthus.

Direct interaction between Hedgehog-sending and Hedgehog-receiving cytonemes is a fundamental mechanism for morphogen transfer and gradient establishment.

Wnt distribution is regulated by a dynamic exchange of its abundant scaffold-bound and rare freely diffusing populations, providing insights into a conceived dilemma of speed and stability of tissue patterning.

A postsynaptic, intracellular, scaffolding protein is necessary to build neuronal gap junctions, revealing an unanticipated complexity of molecular and functional organization of electrical synapses.

Type-I interferon enriched microenvironment generated by Mycobacterium tuberculosis induces the Siglec-1 receptor expression in human macrophages, including on tunneling nanotubes, and contributes to the exacerbation of cell-to-cell transfer of HIV-1.

Fully assembled DNA methylomes from phylogeographically diverse clinical Mycobacterium tuberculosis complex isolates reveals 'intercellular mosaic methylation' as a source of epigenetic diversity.

The CYK-4 subunit of the broadly conserved intercellular bridge component centralspindlin, but not its ZEN-4 subunit, is essential for the cytokinesis-like closure of intercellular bridges that cellularizes oocytes to separate them from germline syncytia.