A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.

Uncoupling of dynamin polymerization and GTPase activity can be induced with dynamin, which allows in vivo visualization of its activity at endocytic pits.

Engineered E3 ubiquitin ligases are utilized to elucidate mechanisms underlying ubiquitin regulation of membrane proteins, and to achieve robust post-translational functional knockdown of ion channels.

In epithelial cells, anillin organizes the medial-apical actomyosin cortex into a contractile load-bearing structure and increases tissue-level stiffness.

New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.

Single episodes of voluntary exercise induced a functional increase in hippocampal synapses mediated by activity-dependent expression of the BAR protein Mtss1L, acting as a novel early effector of synapse formation.

Nanobodies are developed that target neuronal proteins with restricted subcellular distributions and used as intrabodies to deliver cargo to specific sites within neurons and nanoscale immunolabels for improved imaging resolution.

A knockin mouse line was made to label endogenous AMPA receptors, enabling direct observation of synaptic plasticity in behaving animals with single-synapse resolution across the entire brain.

The migrating pair of cardiac progenitors in the chordate Ciona robusta is the simplest possible supracellular cell collective whose organization helps them overcome resistance from surrounding embryonic tissues during cell migration.

Two-color time-lapse superresolution imaging of the synaptic nanoorganization in vivo uncovers highly dynamic spine heads and PSD95 assemblies that are less variable in size but more dynamic in their PSD95 nanoorganization after environmental enrichment.