Christina Chatzi, Yingyu Zhang ... Gary L Westbrook
Single episodes of voluntary exercise induced a functional increase in hippocampal synapses mediated by activity-dependent expression of the BAR protein Mtss1L, acting as a novel early effector of synapse formation.
New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.
A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.
John G Dingus, Jonathan CY Tang ... Constance L Cepko
A large proportion of nanobodies are unstable when expressed intracellularly, but can be reliably stabilized for intracellular expression by applying a generalizable mutagenesis strategy based on positional sequence conservation.
Two-color time-lapse superresolution imaging of the synaptic nanoorganization in vivo uncovers highly dynamic spine heads and PSD95 assemblies that are less variable in size but more dynamic in their PSD95 nanoorganization after environmental enrichment.
Nanobodies are developed that target neuronal proteins with restricted subcellular distributions and used as intrabodies to deliver cargo to specific sites within neurons and nanoscale immunolabels for improved imaging resolution.
Charlotte Rimbault, Christelle Breillat ... Matthieu Sainlos
Engineering new intrabodies allows to develop selective and non-interfering tools for the visualization of endogenous PSD-95 with advanced imaging techniques.
