The cryo-EM structures of actomyosin-V, including a novel strongly bound post-rigor transition state, reveal a pronounced structural heterogeneity of myosin-V and the specific selection of the closed D-loop conformation of F-actin.
NR4A is a broadly conserved transcription factor that is required for concordance of patterning information and anatomy at both ends of the planarian anterior-posterior axis.
Thushani D Nilaweera, David A Nyenhuis, David S Cafiso
Pulse electron paramagnetic resonance on intact Escherichia coli reveals a substrate-induced structural transition in BtuB, the vitamin B12 transporter, which is not seen in reconstituted membranes and indicates a mechanism of transport.
Kayla A Jara, Nikolaus M Loening ... Elisar J Barbar
Autoinhibition by long-range intramolecular interactions in the partially disordered dynein intermediate chain is relieved by multivalent interactions of dynein light chains, demonstrating for the first time the dual roles of light chains in dynein assembly and regulation.
The endosomal apparatus of African trypanosomes consists a continuous membrane system with functional subdomains rather than distinct compartments for early, late, and recycling endosomes.
J Ignacio Gutierrez, Gregory P Brittingham ... Liam J Holt
A combination of single-cell analysis, genomics, and simulations shows that a glutamine-rich low-complexity sequence in the SWI/SNF chromatin remodeling complex senses transient intracellular acidification as a signal for cells to switch their global transcriptional program.
Under systemic iron overload, translational derepression of ferroportin mRNA via the IRE/IRP system antagonizes hepcidin-mediated ferroportin degradation to coordinately control serum iron.
A novel population of hematopoietic cells unmasked by mTORC1 inactivation reveals a new mechanism of innate immune tolerance and a new consequence of defective hematopoiesis.
The initiation of DNA damage checkpoint recovery involves ATM-mediated E3 ubiquitin ligase E6AP phosphorylation which disrupts E6AP-MASTL association and leads to MASTL protein accumulation and cell cycle resumption.
