An atomic model of the 3744-residue Tra1 protein reveals multiple transcription activator binding sites, its integration within the SAGA chromatin coactivator complex, and a striking similarity to DNA-repair factor DNA-PKcs.
Alms1a is a centrosomal protein that exhibits asymmetric localization between mother and daughter centrosomes in asymmetrically dividing stem cells in Drosophila testis, controlling centriole duplication.
Maternal positional information in the fly embryo can be read rapidly in spite of the gene-expression bottleneck and general examples of regulatory architectures that combine speed and accuracy are provided.
A mechanistic basis is provided for the regulative ability of the mammalian embryo offering a long-sought explanation for coordinating cell behaviors at the population level ensuring robustness in developmental outcome.
Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.
Cellularization in Sphaeroforma arctica generates a self-organized structure that morphologically resembles an epithelium, and is associated with tightly regulated expression of cell adhesion pathways.