Microtubule attachment fundamentally modifies kinesin's behavior by triggering a ‘clamshell’ opening of the nucleotide cleft, subsequently reversed by ATP binding, that couples to cargo translocation.
Rosalind Norkett, Urko del Castillo ... Vladimir I Gelfand
Microtubule-microtubule sliding and neurite outgrowth in Drosophila is controlled by a Ser/Thr kinase Tricornered via phosphorylation of a 'mitotic' motor protein Pavarotti.
The first in vitro analysis of full-length human Kif15 provides insight into how hKif15 and its inhibitory binding partner hTpx2 modulate mitotic spindle architecture.
Moirangthem Kiran Singh, Parisa Zangoui ... Linda J Kenney
Genetic code expansion (GCE) enables site-specific labeling and visualization of Salmonella secreted effectors, secretion system components and provides a viable alternative for labeling proteins that do not tolerate N- or C-terminal tags.
Johan OL Andreasson, Bojan Milic ... Steven M Block
A general framework for gating reveals that inter-head tension is not essential for coordinating kinesin stepping, and that neck-linker length is tuned to enhance processivity and velocity.
The structural and functional analysis demonstrates the mechanism of dual functional, motility and microtubule depolymerization in a unique motor, KIF19A.
In quiescent yeast, a stable bundle of parallel microtubules is assembled from the centrosome by an original multistep mechanism that follows a precise temporality and involves Aurora B and various kinesins.
Alex F Thompson, Patrick R Blackburn ... Jason Stumpff
Pathogenic mutations in KIF22, which dominantly cause bone dysplasia, improperly activate the motor during anaphase in dividing cells leading to chromosome recongression.
