Katarzyna Zawadzka, Pawel Zawadzki ... Lidia K Arciszewska
In Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF, a dimeric MukF kleisin binds and activates MukB SMC ATPases through two independent interfaces provided by distinct MukF N- and C-terminal domains.
Habituation to mechanical vibration of crawling Drosophila larvae exhibits distinct time constants that characterize desensitization, re-sensitization, and further adaptation to repeated stimulus patterns.
Drosophila larvae manipulated by an automated system can be observed for 30+ hr, and new analysis methods of long-timescale trajectories reveal behavioral features previously inaccessible, such as a bimodal distribution of thermal navigation efficiency in individual animals.
Yu Takagi, Laurence Tudor Hunt ... Miriam C Klein-Flügge
Adaptation along multiple task-axes reveals choice traces at millisecond resolution in non-invasive recordings from human premotor cortex and provides evidence that theories of top-down control hold even after extensive experience.
Two members of a widely studied family of chemoreceptors, the "orphan" chemoreceptor IR21a and its putative co-receptor IR25a, act together to mediate cool sensation in fruit fly larvae.
Yaara Oppenheimer-Shaanan, Gilad Jakoby ... Tamir Klein
Increased root exudation rate under combined drought and bacterial inoculation and bacterial growth stimulation by specific exudation metabolites support the idea of root recruitment of beneficial bacteria, especially under water stress.
Mechanisms that tether and release replicated sister chromatids to produce sperm and eggs rely extensively on meiotic cohesin complexes that are endowed with unexpectedly different properties specified by a single interchangeable subunit, the α-kleisin.
Meiosis-specific cohesin complexes differing in their kleisin subunit display different chromosome binding dynamics and perform specialised functions in sister chromatid cohesion, chromosome axis organisation, and double-strand break repair.
In vitro assays using Xenopus egg extracts provide evidence that mitosis-specific loading of condensin I is accelerated by phosphorylation of the N-terminal tail of its kleisin subunit.
Chromosome condensation by condensin II is inhibited during interphase by direct interaction with MCPH1, which regulates opening of the condensin II interface between SMC2 and NCAPH2, in a similar fashion to how WAPL regulates Cohesin.