1,875 results found
    1. Chromosomes and Gene Expression
    2. Neuroscience

    Initial elevations in glutamate and dopamine neurotransmission decline with age, as does exploratory behavior, in LRRK2 G2019S knock-in mice

    Mattia Volta et al.
    LRRK2 G2019S knock-in mice are a genetically faithful model that recapitulates the slow disease progression of familial PD, with initial alterations to behaviour and neurotransmission providing early pathophysiological targets for neuroprotective interventions.
    1. Immunology and Inflammation

    Studying the biology of cytotoxic T lymphocytes in vivo with a fluorescent granzyme B-mTFP knock-in mouse

    Praneeth Chitirala et al.
    A new knock-in mouse endogenously labels cytotoxic granules and can be used with all modern super-resolution imaging techniques to study T cell function in vitro and in vivo.
    1. Neuroscience

    Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice

    Srikanta Chowdhury et al.
    Subtraction analysis using novel Orexin-Flp mice isolated physiological importance of orexin peptides.
    1. Neuroscience

    Analysis of SUMO1-conjugation at synapses

    James A Daniel et al.
    Analyses using His6-HA-SUMO1 and SUMO1 KO mice indicate that SUMO1 modification of synaptic proteins is either absent or cannot be detected in brain and cultured neurons.
    1. Cell Biology
    2. Genetics and Genomics

    A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing

    Weina Zhang et al.
    Farrerol promotes the efficiency of CRISPR/Cas9-mediated genome editing in both cells and embryos.
    1. Developmental Biology
    2. Genetics and Genomics

    Efficient targeted integration directed by short homology in zebrafish and mammalian cells

    Wesley A Wierson et al.
    Short homology arms exposed by CRISPR/Cas9 cleavage can target integration at genomic CRISPR/Cas9 cut sites at high frequencies with reproducible precision using pGTag vectors in zebrafish and mammalian cells.
    1. Neuroscience

    Beyond excitation/inhibition imbalance in multidimensional models of neural circuit changes in brain disorders

    Cian O'Donnell et al.
    Computational modeling and analysis of mouse neural population data finds that the excitation/inhibition imbalance theory of brain disorders is too limited to account for key changes in neural activity statistics.
    1. Cell Biology
    2. Developmental Biology

    An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

    Pooran Singh Dewari et al.
    Biochemical tags can be easily knocked into endogenous genes in mammalian stem cells using optimised and scalable protocols that will enable annotation of protein levels, localisation and interaction partners.
    1. Neuroscience

    TUBB4A mutations result in both glial and neuronal degeneration in an H-ABC leukodystrophy mouse model

    Sunetra Sase et al.
    H-ABC leukodystrophy results in neurologic decline, hypomyelination and atrophy of the striatum and cerebellum and Tubb4aD249N/D249Nmouse model recapitulates features of H-ABC disease, providing an important tool for therapeutic development.
    1. Cancer Biology

    Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen

    Hao Zhang et al.
    The HOXA9 reporter and genetic screens facilitated the functional interrogation of the HOXA9 regulome and advanced our understanding of the molecular regulation network in HOXA9-driven leukemia.

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