Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.
BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
Proximity labeling-based proteomic strategies applied in zebrafish identify new insights into protein network changes in heart muscle cells during regeneration and implicate Rho A as a target of ErbB2 signaling during zebrafish heart regeneration.
Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.