3,287 results found
    1. Plant Biology

    Proximity labeling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID

    Andrea Mair et al.
    Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.
    1. Neuroscience

    Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation

    Harrison Tudor Evans et al.
    Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.
    1. Biochemistry and Chemical Biology

    Long-lived metabolic enzymes in the crystalline lens identified by pulse-labeling of mice and mass spectrometry

    Pan Liu et al.
    By performing 15N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.
    1. Neuroscience

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags

    Richi Sakaguchi et al.
    A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.
    1. Neuroscience

    A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

    Réza Shahidi et al.
    A new technique called ‘siGOLD’ allows neural circuits to be mapped using a combination of antibody labeling and electron microscopy.
    1. Cell Biology

    In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish

    Zherui Xiong et al.
    BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.
    1. Developmental Biology

    In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration

    Mira I Pronobis et al.
    Proximity labeling-based proteomic strategies applied in zebrafish identify new insights into protein network changes in heart muscle cells during regeneration and implicate Rho A as a target of ErbB2 signaling during zebrafish heart regeneration.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy

    Sicong He et al.
    Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

    Tino Pleiner et al.
    Protocols that greatly simplify the use of nanobodies in fluorescence imaging and affinity purification have been developed.
    1. Biochemistry and Chemical Biology

    The structural determinants of PH domain-mediated regulation of Akt revealed by segmental labeling

    Nam Chu et al.
    NMR and biochemical analysis reveal new insights into how the critical protein kinase Akt is regulated by phosphorylation and is inhibited by an experimental therapeutic.

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