Proximity-labeling using engineered biotin ligases TurboID and miniTurbo enables detection of cell-type-specific and low abundance protein complexes and subcellular proteomes in Arabidopsis and other plants.

Quantitative de novo proteomics paired with in vivo cell-specific non-canonical amino acid labelling identified several spatial long-term memory-induced changes in protein synthesis in hippocampal neurons.

By performing ¹⁵N pulse-labeling of mice, the turnover of hundreds of proteins in eye tissues was measured by mass spectrometry that revealed long-lived metabolic enzymes in the lens.

A bright and stochastic multicolor labeling method, Tetbow, facilitates millimeters-scale reconstructions of neuronal circuits at a large scale using tissue clearing.

A new technique called ‘siGOLD’ allows neural circuits to be mapped using a combination of antibody labeling and electron microscopy.

BLITZ system enables proximity-dependent biotin labelling in live zebrafish embryos with cell and tissue specificity, providing a versatile and valuable tool for proteomic discovery using the zebrafish model.

Proximity labeling-based proteomic strategies applied in zebrafish identify new insights into protein network changes in heart muscle cells during regeneration and implicate Rho A as a target of ErbB2 signaling during zebrafish heart regeneration.

Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.

Protocols that greatly simplify the use of nanobodies in fluorescence imaging and affinity purification have been developed.

NMR and biochemical analysis reveal new insights into how the critical protein kinase Akt is regulated by phosphorylation and is inhibited by an experimental therapeutic.