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    1. Genetics and Genomics

    One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

    Wenyuan Li et al.
    Conditional gene knockout in combination with fluorescent labeling of different alleles can be achieved with high efficiency in zebrafish through NHEJ-mediated targeted insertion using a CRISPR/Cas system.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy

    Sicong He et al.
    Infrared laser-mediated gene induction microscopy enables precise single-cell labeling in various tissues of zebrafish, and in vivo visualized single-cell lineage tracing reveals the lineage heterogeneity in hematopoietic endothelial cells.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Labeling proteins inside living cells using external fluorophores for microscopy

    Kai Wen Teng et al.
    A toxin is used to introduce an otherwise cell-impermeant fluorophore-antibody (or some thing which is equally specific) to bind to an intracellular protein which allows for super resolution imaging and single particle tracking inside the living cell.
    1. Neuroscience

    FluoEM, virtual labeling of axons in three-dimensional electron microscopy data for long-range connectomics

    Florian Drawitsch et al.
    Methodology to annotate the multiple origins of axonal projections in dense electron microscopy data of mammalian nervous tissue without the need of chemical label conversion is reported.
    1. Cell Biology
    2. Neuroscience

    Specific labeling of synaptic schwann cells reveals unique cellular and molecular features

    Ryan Castro et al.
    The discovery of markers specific to perisynaptic Schwann cells will accelerate the discovery of mechanisms important for their differentiation and function.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

    Tino Pleiner et al.
    Protocols that greatly simplify the use of nanobodies in fluorescence imaging and affinity purification have been developed.
    1. Computational and Systems Biology
    2. Immunology and Inflammation

    T-cell Immunology: The maths of memory

    Jose Borghans, Ruy M Ribeiro
    Mathematical modeling reveals that long-term immunological memory is maintained in a manner that is even more dynamic than previously thought.
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    1. Structural Biology and Molecular Biophysics

    Molecular architecture of human polycomb repressive complex 2

    Claudio Ciferri et al.
    Electron microscopy has been used to produce the first three-dimensional image of the PRC2 gene-silencing complex.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria

    Alam García-Heredia et al.
    Peptidoglycan precursor synthesis occurs in both growing and non-growing regions of the mycobacterial cell surface.