Intracellular growth of the bacterial pathogen Legionella pneumophila in the amoeba Dictyostelium discoideum implicates a bacterial fatty acid transporter as well as dynamic interactions of the distinct membrane-bound replication compartment with host cell lipid droplets.

Multimodal spectroscopy (smFRET, EPR, SAXS, and SANS) and integrative structural modeling reveal large-scale domain rearrangements in human guanylate binding protein 1 (hGBP1) that are driving forces for the formation of oligomers that enable its biological function in innate immune defense.

In potassium-dependent NTPases, insertion of the activating potassium ion into the active site leads to rotation of the gamma-phosphate yielding a near-eclipsed, catalytically productive conformation of the triphosphate chain.

Multicellular and socially aggregating prokaryotes contain previously undescribed, chaperone-based systems predicted to mediate defensive biological conflicts, several components of which are thematically similar antecedents of eukaryotic apoptosis pathways.

Atomic structures suggest a novel mechanism for the regulation of mRNA transcription and capping in dsRNA viruses.

Reengineering the nucleotide-binding pocket of an extant ATPase to restore ancestral GTPase activity revealed an ATP-dependent intermediate required for function and suggested why the protein evolved to use ATP.

Prokaryotic TRADD-N and Death-like adaptor domains in diverse predicted apoptosis and immune systems from multicellular prokaryotes and metazoans indicate the common origin of key apoptosis mechanisms required for the stabilization of multicellularity.

Bacterial Type IV restriction-modification systems display remarkable, previously unnoticed diversity of complex gene and domain architectures, and are predicted to couple antiphage immunity with the abortive infection form of defense.

The GTPase Nog1 orchestrates stalk assembly, peptidyl transferase centre maturation and polypeptide exit tunnel quality control.

Empirical energy landscape allows simulating the structural dynamics of the proteasomal ATPase complex, which yields predictions that are widely consistent with experimental observations and reveals the functional mechanism of the proteasome in substrate degradation.