1,189 results found
    1. Cell Biology
    2. Plant Biology

    Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy

    Sona Valuchova et al.
    Light sheet microscopy was used to establish a method for live imaging of cellular events within Arabidopsis flower and for constructing a 3D model of flower at subcellular resolution.
    1. Cancer Biology
    2. Cell Biology

    Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation

    Ashna Alladin et al.
    The presented primary epithelial acinus-based platform for studying interactions between normal and malignant cells will enable in depth analysis of the tumor initiation process on a molecular level.
    1. Structural Biology and Molecular Biophysics
    2. Developmental Biology

    Cell-accurate optical mapping across the entire developing heart

    Michael Weber et al.
    The combination of high-speed light sheet microscopy and suitable data analysis facilitates cell-accurate measurements across entire organs and opens the way to systematic, scale-bridging, in vivo studies of organogenesis.
    1. Cell Biology
    2. Developmental Biology

    Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy

    Claudia Prahst et al.
    Improved 3D and 4D imaging of neurovascular processes across scales reveals new insights into eye disease mouse models and shows retinal vessels are significantly distorted using standard flat-mount confocal imaging.
    1. Cell Biology

    A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

    Etai Sapoznik et al.
    A novel single-objective light-sheet microscope with unparalleled spatiotemporal resolution enables imaging and optical manipulation of diverse biological specimens and processes.
    1. Cell Biology

    Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding

    Manuel Alonso Y Adell et al.
    Quantitative 3D lattice light sheet microscopy of unperturbed cells combined with electron tomography and acute loss of function experiments reveals how dynamic ESCRT-III/Vps4 assemblies succeed in reverse membrane budding on endosomes.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    3D visualization of macromolecule synthesis

    Timothy J Duerr et al.
    Whole mount 3D visualization of macromolecule synthesis with light sheet fluorescence microscopy enables quantitative, multiscale analysis at the organ, cellular, and molecular levels of organization.
    1. Developmental Biology
    2. Chromosomes and Gene Expression

    A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines

    Frederic Strobl et al.
    In transgenesis assays involving diploid model organisms, two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs allow the systematic creation of homozygous transgenic lines and minimize the number of wasted animals.
    1. Microbiology and Infectious Disease

    Mechanisms of virus dissemination in bone marrow of HIV-1–infected humanized BLT mice

    Mark S Ladinsky et al.
    Large-volume light microscopy combined with higher-resolution electron tomography revealed the spatial distribution of virus-producing cells and highlighted mechanisms of HIV-1 dissemination in bone marrow from a small animal model.
    1. Physics of Living Systems

    Heterogeneous T cell motility behaviors emerge from a coupling between speed and turning in vivo

    Elizabeth R Jerison, Stephen R Quake
    Motile interstitial T cells in live zebrafish access a broad range of length-scales due to long-lived cell-intrinsic variation in speed, and a coupling between speed and directional persistence.

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