eMags is an engineered photodimerizer pair for optogenetic modulation in mammalian cells that is especially suited for the manipulation of intracellular processes occurring in small volumes or subcellular organelles.

The cancer-associated human ubiquitin ligase HUWE1 can adopt an auto-inhibited dimeric state, whose occupancy is regulated by competing intra- and intermolecular interactions of the dimerization region and by the tumor suppressor p14ARF.

A single protein catalyzes site-specific DNA recombination upon blue-light illumination.

Doublecortin activates its C-terminal domain when its N-terminal domain engages the microtubule lattice, leading to self-associations that explain the stabilizing effect of Doublecortin in neuronal migration.

Single-molecule TIRF microscopy, biochemical assays and mathematical modeling demonstrate how actin-branch nucleation by the Arp2/3 complex is coordinately regulated by two biochemically distinct activators.

Synthetic cytokines engage new signaling programs with therapeutic potential.

A mechanistic dissection of how autophagy kinase Atg1 is activated in fission yeast reveals surprising differences from the known Atg1 activation mechanisms previously defined in budding yeast.

LAMP proteins, the major glycoproteins of the lysosome membrane, bind cholesterol directly and specifically, and interact with NPC1 and NPC2 proteins as part of the lysosomal cholesterol export process.

The engagement of DNA crossings is shown to license ATP hydrolysis and DNA cleavage by topoisomerase VI, a finding with mechanistic ramifications for related GHKL ATPases and meiotic recombination machineries.