Marius Landau, Sherif Elsabbagh ... Joachim E Schultz
The dodecahelical membrane anchors of mammalian adenylyl cyclase isoforms are receptors for unsaturated free fatty acids and, as a tonic signal, enhance or attenuate GPCR/Gsα-stimulated cyclic AMP formation.
Lakshmi E Miller-Vedam, Bastian Bräuning ... Jonathan S Weissman
Structure-function characterization of the EMC's cytoplasmic, transmembrane, and lumenal domains reveal features critical for terminal helix insertion and a specialized role for the lumenal domain in polytopic membrane protein biogenesis.
In parallel to protein-driven processes, characteristic physicochemical properties of the endoplasmic reticulum membrane modulate intracellular fat accumulation and lipid droplet formation.
Inês Gomes Castro, Shawn P Shortill ... Maya Schuldiner
High-throughput analysis of several Saccharomyces cerevisiae contact sites reveals a large data set of potential contact site proteins, including proteins involved in the regulation of lipid distribution within cells.
A high-resolution method to quantify interactions between lipid bilayers and single proteins under controlled load is presented and applied to key proteins involved in membrane fusion and formation and maintenance of membrane contact sites.
Rigorous assays of membrane fusion show that a distinct tethering step is required for lumenal compartment mixing in a manner that extends beyond simply increasing the amount of total trans-SNARE complex.
MakA, a pore-forming cytotoxin produced by Vibrio cholerae, forms oligomers and remodels membranes into high-curvature tubes, resulting in membrane integrity loss inside acidified organelle lumens or when cultured with cells in an acidic medium.
Birol Cabukusta, Shalom Borst Pauwels ... Jacques Neefjes
Lipid transfer proteins at the ER-Golgi interface synchronize intracellular lipid flows through the secretory pathway, ensuring the asymmetric lipid composition of the plasma membrane.
