Madhurima Dhara, Maria Mantero Martinez ... Dieter Bruns
Naturally-occurring v-SNARE TMD variants differentially regulate fusion pore dynamics and cooperate with phospholipids in supporting membrane curvature at the fusion pore neck.
Florian Wilfling, Abdou Rachid Thiam ... Tobias C Walther
The targeting of proteins from the endoplasmic reticulum to lipid droplets relies on surface modulation of the lipid droplet by the Arf1/COPI machinery.
Carl Leung, Natalya V Dudkina ... Bart W Hoogenboom
Electron and atomic force microscopy show how bacterial toxins bind to a host membrane and assemble into arcs and rings, before undergoing a dramatic, concerted conformational change to insert into the membrane.
Yashar Sadian, Christos Gatsogiannis ... Stefan Raunser
Imaging with electron microscopy and cryo-electron tomography reveals that competition between Cdc42 and Gic1 for the same subunit within septins controls the formation and breakdown of septin filaments.
Calcium-binding synaptotagmins are involved in ring oligomer formation, which allows synaptotagmins to synchronize neurotransmitter release to Ca2+ influx.
Association of curvature generating proteins to the Golgi membranes by sphingomyelin metabolism essentially controls the flatness of a Golgi cisterna that is necessary for efficient sorting and export.
The first de novo synthesis of polyisoprenoids correspond to natural rubber in vitro with the recombinant proteins is achieved by identification and cell-free translation-coupled reconstitution of the rubber biosynthetic machinery on rubber particles.
Manuel Giménez-Andrés, Tadej Emeršič ... Alenka Čopič
The amphipathic helix of perilipin 4 relies on the organization of its polar residues to form a remarkably immobile and stable protein layer on the surface of lipid droplets.
A pushing force on the ankyrin repeat region of NompC leads to the gating of the mechanosensitive ion channel, which may enable cells to feel compression and shrinkage.
