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Analysis of periprotein lipids by direct extraction of membrane proteins, using styrene-maleic acid polymers.

We challenge a recent report that most of the cholesterol in the plasma membrane of mammalian cells is in the exofacial leaflet.

The golgin GMAP-210 captures vesicles at the cis Golgi by merely recognizing the high curvature and lipid unsaturation level of transport vesicles.

Mining electron cryomicroscopy data reveals new ribosomal interactions that preclude translation to potentially regulate gene expression.

Cryo-electron tomography, reconstitution, and electrophysiological data show that a fundamental function of Munc13-1 is to bridge synaptic vesicles to the presynaptic plasma membrane.

Munc13 C-terminal domains synergize to coordinate synaptic vesicle docking, priming and fusion.

Within minutes after an abrupt increase in membrane tension, yeast cells reduce their membrane tension by modulating their rate of endocytosis and exocytosis, and adapt their endocytic actin machinery.

Although puromycin staining is often used to examine subcellular translation, puromycin-labeled proteins are rapidly released from ribosomes even in the presence of elongation inhibitors, which may confound translation site localization.

MakA, a pore-forming cytotoxin produced by Vibrio cholerae, forms oligomers and remodels membranes into high-curvature tubes, resulting in membrane integrity loss inside acidified organelle lumens or when cultured with cells in an acidic medium.

Genome dilution limits cell growth by modulating the activities, rather than the concentrations, of RNA polymerases and ribosomes, and is accompanied by changes in proteome composition.