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    1. Biochemistry and Chemical Biology
    2. Cell Biology

    A subcellular map of the human kinome

    Haitao Zhang et al.
    An immunofluorescent analysis of 465 protein kinases revealed a substantial role of liquid-liquid phase separation in kinase localization and identified new kinase localizations to mitochondria and other organelles.
    1. Physics of Living Systems

    Single molecule microscopy reveals key physical features of repair foci in living cells

    Judith Miné-Hattab et al.
    Upon DNA damage, Rad52 forms a membraneless sub-compartment where Rad52 molecules are highly dynamic and share properties with liquid-liquid phase-separated molecules, reflecting the existence of a liquid droplet around damaged DNA.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA

    Ruchika Sachdev et al.
    The protein Pat1 functions in the assembly of processing bodies, cellular membraneless organelles, by promoting the liquid–liquid phase separation of the DEAD-box ATPase Dhh1 and RNA.
    1. Physics of Living Systems
    2. Structural Biology and Molecular Biophysics

    Dynamic metastable long-living droplets formed by sticker-spacer proteins

    Srivastav Ranganathan, Eugene I Shakhnovich
    Multi-scale simulations reveal a potential, ATP-independent mechanism resulting in the formation of the long-living multi-droplet state by multi-valent, spacer-sticker proteins.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

    David Trombley McSwiggen et al.
    Transient DNA interactions by DNA-binding proteins are utilized by herpes simple virus as an alternative route to generate membraneless compartments in the nucleus without invoking phase separation.
    1. Structural Biology and Molecular Biophysics

    Pre-transition effects mediate forces of assembly between transmembrane proteins

    Shachi Katira et al.
    A general mechanism for how powerful forces of assembly and protein mobility can emerge from phase transitions is established.
    1. Neuroscience

    SynGAP isoforms differentially regulate synaptic plasticity and dendritic development

    Yoichi Araki et al.
    Detailed SYNGAP1 splice variant characterization uncovers distinct isoform functions and biochemical properties contributing to critical aspects of neurodevelopment, providing previously unknown isoform-level insight into SYNGAP1-related cognitive disorders.
    1. Immunology and Inflammation

    Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes

    Matthew B Stone et al.
    Clustering the B cell receptor generates a membrane domain analogous to the liquid-ordered phase, localizing proteins involved in early receptor activation.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules

    Sonja Kroschwald et al.
    A comparison in yeast and human cells reveals a remarkable variability in the properties of RNP granules and highlights a key role for protein disaggregation machines in regulating RNP granule assembly.
    1. Cell Biology

    CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets

    Jordina Guillén-Boixet et al.
    CPEB4's switch from translational repressor to activator is regulated during cell cycle by hyperphosphorylation of its intrinsically disordered domain, which controls its phase-separation into RNA-containing liquid-like droplets.