Ando Christian Zehrer, Ana Martin-Villalba ... Helge Ewers
High-quality fluorescence live-cell and single molecule imaging via computer-controlled, user-assembled microscope that fits in incubator, can in large parts be 3D printed and employs open source software and electronics.
Sox2 transcription is not correlated with spatial proximity of its essential regulatory enhancer in embryonic stem cells, suggesting gene transcription is not limited to periods of direct enhancer-promoter contact.
Light sheet microscopy was used to establish a method for live imaging of cellular events within Arabidopsis flower and for constructing a 3D model of flower at subcellular resolution.
Iodixanol supplementation provides a simple method for tuning the refractive index of live imaging media, which can greatly improve resolution and penetration depth in live imaging experiments.
A robust fluorescence microscopy-based data acquisition and analysis framework affords the precise measurement of cell surface receptor affinities toward their cognate ligands and their densities in live cells/tissues.
The existence of a slow-moving zone around the transcription site may unify conflicting models of co-transcriptional versus post-transcriptional mRNA splicing.
Alain Pulfer, Diego Ulisse Pizzagalli ... Santiago Fernandez Gonzalez
ADeS, a novel deep learning approach for apoptosis detection, achieves 98% accuracy in vitro and in vivo while providing unprecedented insights into the spatial–temporal foundation of cell death.
Saskia MA de Man, Gooitzen Zwanenburg ... Renée van Amerongen
Genome editing, advanced live-cell microscopy, and computational modeling were combined to measure WNT/CTNNB1 signaling parameters at the single molecule level, revealing critical regulatory nodes in this important signal transduction pathway.
Franck Simon, Maria Colomba Comes ... Herve Isambert
CausalXtract is a computational tool to discover causal effects, beyond classical Granger and Schreiber causality approaches, from live-cell imaging data for a range of fundamental and translational research applications.
Nuclear pores assemble asymmetrically, by an inside-out evagination of the inner nuclear membrane that grows in diameter and depth until it fuses with the flat outer nuclear membrane.
