Single molecular imaging of MeCP2 at high spatial/temporal resolution identifies distinct structural domains contributing to its dynamic behaviors and chromatin interactions in live neurons.
The main proteins of clathrin-mediated endocytosis bind and unbind rapidly, continuously turning over about five times during the formation of an endocytic vesicle in yeast.
Anders S Hansen, Maxime Woringer ... Xavier Darzacq
Spot-On is an easy-to-use website that makes a rigorous and bias-corrected modeling framework for analysis of single-molecule tracking experiments available to all.
Lattice light-sheet single-molecule imaging shows 3D Sox2 enhancer clusters in live embryonic stem cells and reveals a model linking 3D spatial distribution of cis-elements, differential target search features and localized gene regulation.
Histone variant H2A.Z is deposited near transcription start sites by the chromatin remodeler SWR1 and seems to be removed by RNA polymerase II at an early stage of transcription elongation.
Ayhan Atmanli, Dongjian Hu ... Ibrahim John Domian
A novel live-cell mRNA imaging technology identifies individual living cells based on their gene expression and enables the concurrent study of their physiology.
Ignacio Izeddin, Vincent Récamier ... Xavier Darzacq
While the transcription factor c-Myc explores the space in the nucleus in an unrestricted manner, the elongation factor P-TEFb's sampling of the nucleus is constrained to a complex domain with fractal characteristics.
The existence of a slow-moving zone around the transcription site may unify conflicting models of co-transcriptional versus post-transcriptional mRNA splicing.
