Nanobodies are developed that target neuronal proteins with restricted subcellular distributions and used as intrabodies to deliver cargo to specific sites within neurons and nanoscale immunolabels for improved imaging resolution.

New intracellularly-expressed llama anti-EPS15 nanobodies reveal that pioneer clathrin-coat components function upstream of AP-2 clathrin adaptor deposition at the plasma membrane.

A genetic screen has identified the first signaling component of the unfolded protein response in chloroplasts.

A tumor-specific regulatory region has been identified upstream of the Myc oncogene.

High resolution structures of the essential human AAA+ ATPase TorsinA and its disease mutant in complex with an activator reveal details of the interaction that will guide drug design and further functional characterization.

LAP1 adopts an AAA+ like fold that, while unable to bind nucleotide, can enhance ATPase activity in the neighboring TorsinA protomer in an unusual heterohexameric ring, via an arginine finger.

X-ray crystallography reveals how kinesin-1 recognises a novel class of cargo adaptor motifs in an isoform-specific manner.

Uncoupling of dynamin polymerization and GTPase activity can be induced with dynamin, which allows in vivo visualization of its activity at endocytic pits.

Inside the acidic vacuole, expression, DNA binding and DNA binding affinity of the Salmonella regulator SsrB is stimulated by acid pH.