The structure of the Nef:AP-2 complex has been determined and used as the basis of a model that explains how HIV-1 Nef downregulates the CD4 receptor from the surface of the infected cells.

LAP1 adopts an AAA+ like fold that, while unable to bind nucleotide, can enhance ATPase activity in the neighboring TorsinA protomer in an unusual heterohexameric ring, via an arginine finger.

Rab6A binds directly to both the C-terminus and the N-terminal motor domain of the kinesin KIF1C to regulate vesicle motility and Golgi organization.

A new family of sterol-specific lipid transfer proteins has been found that anchors in the endoplasmic reticulum; some of these proteins stretch across membrane contacts and mediate sterol traffic from the plasma membrane.

The C. elegans ninein homolog NOCA-1 is important for the assembly of non-centrosomal microtubule arrays required for embryo production, nuclear positioning and larval development.

Protocols that greatly simplify the use of nanobodies in fluorescence imaging and affinity purification have been developed.

PHF13 interacts with transcriptional complexes containing RNA polymerase II to recruit/stabilize them at H3K4me2/3 containing active or bivalent promoters.

A straightforward, rapid and generalizable strategy uses modified nanobodies to convert intracellular protein detection into desired biological activities in cells and animals.

A fully synthetic library of humanized single domain antibodies yields in vitro high affinity antibodies usable in cell biology and translational projects.