Genome-wide profiling of R-loops at near single-nucleotide resolution reveals distinct R-loop boundaries depending on the presence and location of the first exon-intron junction.

Certain types of 3D chromatin loops are easy to predict from existing or easily obtainable 2D information, which benefits gene expression studies in tissues/cells/organisms without extensive pre-existing 3D information.

Genetic analyses in budding yeast coupled with biochemical assays have revealed an unexpected role for the Smc5/6 complex in cellular pathways responsible for the prevention of RNA formation in genomic DNA.

BisMapR reveals strand-specific R-loops with high resolution at small-scale genomic features including bidirectionally transcribed promoters and enhancers.

CTCF protects cohesin^STAG1 from WAPL.

Seemingly contradictory findings of single-molecule and in vivo experiments on a major mechanism of chromosome organization are reconciled by computationally investigating mechanisms of loop extrusion that are consistent with both.

The deposition of 5-hydroxymethylcytosine epigenetic marks in active genes promotes the annealing of the nascent RNA to the template DNA strand, forming an R-loop.

High-resolution mapping of cohesin-dependent chromatin loops in the genome of budding yeast reveals evolutionarily conserved features for loop formation and cohesin residency as a determinant of loop positioning.

Boundary:boundary pairing generates either stem-loop or circle-loop TADs, and these loop topologies differ in how they impact chromosome structure and genetic activities.

Large regions of foreign DNA inserted into mammalian chromosomes assemble into H3K9me3-heterochromatin and result in increased condensin loading with organisation into shorter mitotic chromosome loops than endogenous DNA.