Dhh1, Pat1, and Lsm1 target subsets of cellular mRNAs for decapping via interactions of these regulatory proteins with the C-terminal domain of Dcp2, the catalytic component of the decapping enzyme.
Eukaryotic mitochondrial RNA polymerases cap RNA with NAD with much higher efficiencies than nuclear RNA polymerase II; as a consequence, mitochondrial RNAs have remarkably high levels of NAD capping.
Crosslink immunopreciptiation (iCLIP) studies reveal important mechanistic insights into how MARF1 post-transcriptionally regulates targeted mRNAs and uncover a novel mode by which EDC4 regulates mRNA metabolism.
mRNA decapping function in neuronal cells regulates neurosecretion and intertissue signaling, affecting developmental and ageing processes in two model organisms.
A multidimensional chemical mapping strategy enables confident determination of the structures of non-coding RNAs at 1-nm resolution, including previously intractable riboswitch and human regulon states.
Mapping of 30 general RNA degradation factors onto the yeast transcriptome provides the global distribution of factors for RNA turnover and surveillance in a eukaryotic cell.
Post-translational modification of histone H3K36 is not required to suppress cryptic transcription initiation or to include alternative exons in Drosophila; instead it promotes expression of active genes by stimulating polyadenylation.
Structural and functional studies reveal how Newcastle disease virus nucleocapsid protects its viral genome through a self-capping mechanism, which is important for new antiviral drug design.
