Single molecule mRNA imaging uncovers post-transcriptional regulation of myc mRNA, via a cell-intrinsic mechanism allowing individualised control of neural stem cell proliferation during Drosophila brain development.
Genomic-profiles and reporters reveal that the three-nucleotide ‘words’ read by the ribosome, codons, have a strong effect on mRNA stability, impacting the homeostatic mRNA and protein levels in human cells.
A c-Myc-transcribed long noncoding RNA namely LAST (LncRNA-assisted stabilization of transcripts) collaborates with a cellular factor CNBP to promote the stability of CCND1/cyclin D1 mRNA post-transcriptionally, ensuring the proper G1/Sphase transition of the cell cycle.
In the torpid hibernator, when transcription is inhibited, enhanced stability and polyadenylation explain increased abundance of crucial transcripts required for intense non-shivering thermogenesis during arousal.
For poly(A)-tail length to influence mRNA translational efficiency, poly(A)-binding protein (PABPC) must be limiting, mRNAs lacking PABPC must be stable, and translation initiation must be sensitive to PABPC levels.