Single molecule mRNA imaging uncovers post-transcriptional regulation of myc mRNA, via a cell-intrinsic mechanism allowing individualised control of neural stem cell proliferation during Drosophila brain development.
Non-invasive mRNA stability measurements reveal that transcript lifetime is governed by a competition with translation initiation on a transcriptome-wide level.
Genomic-profiles and reporters reveal that the three-nucleotide ‘words’ read by the ribosome, codons, have a strong effect on mRNA stability, impacting the homeostatic mRNA and protein levels in human cells.
Selective degradation of mature miRNAs shapes temporal miRNA expression patterns and is important for proper regulation of target genes to support normal development of Drosophila embryos.
A c-Myc-transcribed long noncoding RNA namely LAST (LncRNA-assisted stabilization of transcripts) collaborates with a cellular factor CNBP to promote the stability of CCND1/cyclin D1 mRNA post-transcriptionally, ensuring the proper G1/Sphase transition of the cell cycle.
Interaction with PUMILIO is essential for maintenance of genomic stability by the cytoplasmic long noncoding RNA NORAD, whereas binding to RBMX is dispensable for this function.
When a protein involved in DNA repair malfunctions, it can anneal RNA molecules to DNA molecules, creating hybrids that increase the frequency of mutations in the DNA.
In the torpid hibernator, when transcription is inhibited, enhanced stability and polyadenylation explain increased abundance of crucial transcripts required for intense non-shivering thermogenesis during arousal.
For poly(A)-tail length to influence mRNA translational efficiency, poly(A)-binding protein (PABPC) must be limiting, mRNAs lacking PABPC must be stable, and translation initiation must be sensitive to PABPC levels.
