Reconstitution of dynein-based mRNA transport with purified components reveals a pivotal role of an RNA localisation signal in controlling the assembly and activity of the translocation machinery.
mRNA cargo orchestrates the activation of an in vitro reconstituted dynein-dynactin-Bicaudal D-Egalitarian complex for robust processive motion on microtubules.
An endosomal component employs a novel PAM2-like motif to recruit a key RNA-binding protein, which explains how mRNAs and associated ribosomes are attached to endosomes during coupled transport.
Expanded repeat RNAs associated with human neurodegenerative diseases can become incorporated into transported granules in neurons, perturbing their function to cause neuritic branching defects.
The spatial regulation of gene expression within neurons occurs primarily at the level of local translation rather than by stimulus-induced RNA targeting from nucleus to synapse.
Dynactin acts as an anti-catastrophe factor that extends microtubule growth; posteriorly elucidating a new essential step in oskar mRNA localisation and providing a novel mechanism by which motor-dependent transport can amplify the polarity of MT networks.
Cellular acidity, capacity for net acid extrusion, and expression of acid-base transporters in human breast carcinomas independently predict variation in proliferative activity, lymph node metastasis, and patient survival.
In vitro reconstitution of mRNA motility reveals the basis of directionally biased motion by groups of motors, their response to potential obstacles, and the consequences of reaching microtubule ends.
A three-dimensional description of the cytoskeletal arrangement, cytoplasmic flows, and cargo transport in stage 9 Drosophila oocytes accurately reproduces mRNA localizations in wild-type and mutant oocytes.
