A modified form of Green Fluorescent Protein integrated into an ammonium transporter provides a sensor that can be used to monitor transport activity in vivo.
Experiments on E. coli show that multiple mechanisms contribute to extreme resistance to ionizing radiation in bacteria, with mutations to three genes for DNA repair having a prominent role in one evolved population.
Increasing levels of atmospheric carbon dioxide reduce the mineral content but increase the levels of starch and sugars found in crop plants; which could exacerbate both obesity and malnutrition in some human populations.
Alessandro Costa, Ludovic Renault ... James M Berger
The Mcm2-7 motor unwinds DNA using an approach distinct from that of superfamily III helicases, and accesses multiple ring configurations and assembly states during the initiation of DNA replication.
Tobias Willer, Kei-ichiro Inamori ... Kevin P Campbell
Post-phosphoryl modification of α-dystroglycan requires the glucuronyltransferase B4GAT1; this enzyme synthesizes the acceptor glycan that serves as a primer for the glycosyltransferase LARGE to synthesize the laminin-binding glycan.
Nicholas A Bokulich, Jordyn Bergsveinson ... David A Mills
Mapping microbial landscapes in indoor environments can predict how contaminants and spoilage resistance genes propagate within food-production environments, yielding novel insight for controlling spoilage.