To facilitate human developmental biology research, CRISPR-mediated homologous recombination, tightly inducible gene knockdowns (CRISPRi) and overexpression (CRISPRa) have been efficiently applied to human organoids.
Genetic disruption of sodium-hydrogen exchanger 6 (NHE6) reduces amyloid plaques in humanized Alzheimer's disease mouse models and restores normal synaptic responses to neuromodulatory input in humanized ApoE4-expressing animals.
Despite evidence of significant anti-cryptococcal activity in vitro and animal models, including synergy with other antifungal agents, high-dose tamoxifen has no impact on cerebrospinal fluid sterilization in cryptococcal meningitis.
Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.
Fluorescence fluctuation spectroscopy is implemented for detection of up to four molecular species, allowing users to quantify molecular interactions and stoichiometry of multicomponent complexes in live cells, in a wide range of biological processes, from membrane signaling to viral assembly.