Neurons differ in their impact on collective cortical activity, with sensitive neurons forming a stable topological core, implicated in cortical-state transitions, while peripheral insensitive neurons are more responsive to stimuli.
Unbiased and automatic annotation using structured prediction framework with efficiently built data-driven atlases is more accurate than registration-based methods for cell identifications in dense images and enables fast whole-brain analysis.
Association of curvature generating proteins to the Golgi membranes by sphingomyelin metabolism essentially controls the flatness of a Golgi cisterna that is necessary for efficient sorting and export.
Advanced microscopy techniques reveal that clusters of Pom1 kinase at the membrane represent the functional unit that shape the concentration gradients and modulate Pom1 mid-cell levels according to cell size.
Structural and functional analyses show that transverse tubule association with stacks of sarcoplasmic reticulum enhances Orai1-dependent Ca2+ entry to replenish Ca2+ stores, maintain Ca2+ release, and maximize force during exercise.
Computer simulations of the evolution of broadly neutralizing antibodies against HIV suggest that non-traditional pathways involving framework mutations which lead initially to increased antibody flexibility do occur, but can be avoided by appropriate vaccine design.