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    1. Chromosomes and Gene Expression

    A multiplexed DNA FISH strategy for assessing genome architecture in Caenorhabditis elegans

    Brandon D Fields, Son C Nguyen ... Scott Kennedy
    The design and validation of an oligo-based DNA FISH library that targets the entire Caenorhabditis elegans genome at chromosome, three megabase, and 500 kb scales.
    1. Epidemiology and Global Health
    2. Genetics and Genomics

    Environmentally sensitive hotspots in the methylome of the early human embryo

    Matt J Silver, Ayden Saffari ... Andrew M Prentice
    259 CpGs associated with season of conception in rural Gambians constitute a valuable resource for investigating epigenetic mechanisms linking early exposures to lifelong health and disease.
    1. Developmental Biology
    2. Neuroscience

    Caenorhabditis elegans processes sensory information to choose between freeloading and self-defense strategies

    Jodie A Schiffer, Francesco A Servello ... Javier Apfeld
    C. elegans nematodes use a sensory-neuronal circuit to determine whether to defend themselves from hydrogen peroxide attack or to freeload off orthologous protective defenses from bacteria in their surrounding environment.
    1. Stem Cells and Regenerative Medicine

    AIRE is a critical spindle-associated protein in embryonic stem cells

    Bin Gu, Jean-Philippe Lambert ... Janet Rossant
    AIRE played novel roles in the mitosis process in embryonic stem cells and early embryo cells.
    1. Chromosomes and Gene Expression

    Epigenetic reprogramming by TET enzymes impacts co-transcriptional R-loops

    João C Sabino, Madalena R de Almeida ... Sérgio Fernandes de Almeida
    The deposition of 5-hydroxymethylcytosine epigenetic marks in active genes promotes the annealing of the nascent RNA to the template DNA strand, forming an R-loop.
    1. Cell Biology

    A single-cell transcriptome atlas of pig skin characterizes anatomical positional heterogeneity

    Qin Zou, Rong Yuan ... Yanzhi Jiang
    The heterogeneity in molecule and function of three main cell types of smooth muscle cells, endothelial cells, and fibroblasts from both different anatomical sites of pig skin was comprehensively exposited.
    1. Developmental Biology

    Distinct functions of three Wnt proteins control mirror-symmetric organogenesis in the C. elegans gonad

    Shuhei So, Masayo Asakawa, Hitoshi Sawa
    The mirror-symmetric structure of the Caenorhabditis elegans gonad is established through the Wnt-independent function of a Frizzled protein and three Wnt proteins with distinct roles.
    1. Developmental Biology

    A complex regulatory landscape involved in the development of mammalian external genitals

    Ana Rita Amândio, Lucille Lopez-Delisle ... Denis Duboule
    In vertebrates, large regulatory landscapes sometimes behave as coherent regulatory units, which may explain the lack of effect sometimes observed when single enhancer sequences are deleted in isolation.
    1. Chromosomes and Gene Expression

    The Smc5/6 complex counteracts R-loop formation at highly transcribed genes in cooperation with RNase H2

    Shamayita Roy, Hemanta Adhikary ... Damien D'Amours
    Genetic analyses in budding yeast coupled with biochemical assays have revealed an unexpected role for the Smc5/6 complex in cellular pathways responsible for the prevention of RNA formation in genomic DNA.
    1. Cell Biology
    2. Physics of Living Systems

    The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy

    Akanksha Thawani, Michael J Rale ... Sabine Petry
    Four αβ-tubulin dimers associate laterally on γ-TuRC to define the rate-limiting transition state for microtubule nucleation.