The requirement for WNT signaling in mesendoderm differentiation is temporally separate from that of ACTIVIN signaling and acts to switch the output of ACTIVIN/SMAD2 from pluripotency maintenance to mesendoderm patterning.

Adding suppressors of spontaneous differentiation enables precise control of human induced pluripotent stem cell (hiPSC) status in suspension culture conditions and leads to scalable and automated cell therapy using hiPSCs.

Genomic analysis of Xenopus gastrula reveal that the transcription factor Sox17 interacts with the Wnt signaling effector ß-catenin on enhancers to regulate the transcriptional program underlying endoderm germ layer formation.

Different combinations of the transcription factors Pou5f3, Nanog and Sox32 form complexes to drive dorsoventral mesendoderm patterning in zebrafish.

Ascidian mesendoderm is a transient regulatory state upstream of mesoderm and endoderm gene regulatory networks and required for the initiation of both.

Human yolk sac-like cells, which share characteristics with the post-implantation human hypoblast, can model the interaction between the epiblast and hypoblast that occurs during early human development.

Dynamic control of intrinsic pluripotent multicellular self-organization to yield robust symmetry breaking patterns that recapitulate morphogenic processes associated with developmental events.

The Apelin receptor acts as a rheostat to ensure that the proper levels of Nodal signaling are achieved for proper cell fate specification at the onset of gastrulation, in particular for cardiac progenitor development.

Investigation of defined extracellular matrix proteins in differentiation of human pluripotent stem cells to cardiomyocytes reveals fibronectin, in addition to soluble factors, is required for cardiogenesis.

Genetic analyses revealed the remarkable plasticity of mesodermal cells to differentiate across the germ layers to form insulin-producing β-cells, which are normally of endodermal origin.