As the first fully genetically encoded method, PARIS allows cell-specific, long-term, repeated measurements of gap junctional coupling with high spatiotemporal resolution, facilitating its study in both health and disease.
A simple, yet elegant method for robust self-assembly of diverse membrane proteins into soluble peptide nanoparticles for their structural and functional analysis in detergent-free solutions.
Without using control cohorts, a statistical method identifies candidates for immune receptors associated with diseases from repertoire sequencing datasets.
A new technique for biodistribution and targeted uptake studies of nanoparticle imaging agents is demonstrated and used to achieve levels of detail that are inaccessible through existing methods.
An optimized 3D fluorescence co-localization method is a useful toolkit to obtain cellular 3D separations between green and red labeled protein domains with nanometer-scale accuracy using light microscopy.
A method for determining how many ions are required to drive transport in an electrogenic secondary active transporter will help understand the mechanisms of bacterial transporters whose structures have been solved.
A new high-throughput method for single-cell RNA-seq in yeast cells shows how stochastic expression of glucose-repressed genes contributes to cell-to-cell differences during adaptation to an environmental change.
Hamilton's rule can be violated when costs and benefits of cooperation are defined using the counterfactual method, and when they depend on the cooperation of others.
New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.
