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    1. Evolutionary Biology

    Can Hamilton’s rule be violated?

    Matthijs van Veelen
    Hamilton's rule can be violated when costs and benefits of cooperation are defined using the counterfactual method, and when they depend on the cooperation of others.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps

    Abhishek Singharoy et al.
    New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.
    1. Developmental Biology
    2. Neuroscience

    Revisiting the role of Dcc in visual system development with a novel eye clearing method

    Robin J Vigouroux et al.
    A new eye-specific Dcc mutant combined with an improved clearing protocol for the eye and brain (EyeDISCO) reveals the requirement of the receptor Dcc for retinal development and maintenance.
    1. Genetics and Genomics
    2. Neuroscience

    A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes

    François Kroll et al.
    Zebrafish knockouts can be generated in a few hours directly from wild-type eggs and are suitable for studying continuous traits, including behaviour.
    1. Neuroscience

    Improving a probabilistic cytoarchitectonic atlas of auditory cortex using a novel method for inter-individual alignment

    Omer Faruk Gulban et al.
    Inter-individual human brain alignment that uses macro-anatomical priors in addition to cortical curvature improves micro-anatomical correspondence between auditory areas.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A systematically-revised ribosome profiling method for bacteria reveals pauses at single-codon resolution

    Fuad Mohammad et al.
    With the removal of confounding artifacts, ribosome profiling can yield insight into the mechanism of protein synthesis in bacteria at high resolution.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells

    Chitra Mohan et al.
    An in vivo disulfide crosslinking assay shows preferential disassembly of nucleosomes with two H2A.Z histones by transcription machinery in yeast and conjugation to one or two ubiquitin moieties in human cells.
    1. Chromosomes and Gene Expression
    2. Neuroscience

    Conditional protein tagging methods reveal highly specific subcellular distribution of ion channels in motion-sensing neurons

    Sandra Fendl et al.
    New protein labeling strategies unravel the subcellular distribution of neurotransmitter receptor subunits and voltage-gated ion channels in motion-sensing T4/T5 neurons of the Drosophila visual system.
    1. Neuroscience

    A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes

    Réza Shahidi et al.
    A new technique called ‘siGOLD’ allows neural circuits to be mapped using a combination of antibody labeling and electron microscopy.
    1. Chromosomes and Gene Expression

    A simple method for generating high-resolution maps of genome-wide protein binding

    Peter J Skene, Steven Henikoff
    Building on previous work (Skene et al., 2014), we show that a new ChIP-seq protocol provides superior resolution and ease of use at low sequence depth of coverage for generating genome-wide maps of protein binding.