RNA-binding protein SRSF3 mediates critical changes in RNA processing of pluripotency genes, which reveals functional consequences of regulated RNA processing during stem cell self-renewal and early development.
Selective degradation of mature miRNAs shapes temporal miRNA expression patterns and is important for proper regulation of target genes to support normal development of Drosophila embryos.
Single molecule mRNA imaging uncovers post-transcriptional regulation of myc mRNA, via a cell-intrinsic mechanism allowing individualised control of neural stem cell proliferation during Drosophila brain development.
The RNA-binding protein PTBP1 is recruited to sites near stop codons in retroviral and human mRNAs, shielding them from detection and degradation by the nonsense-mediated mRNA decay pathway.
Genomic-profiles and reporters reveal that the three-nucleotide ‘words’ read by the ribosome, codons, have a strong effect on mRNA stability, impacting the homeostatic mRNA and protein levels in human cells.
Excess expression of the Gadd45 mRNA accounts for lethality when nonsense-mediated decay is lost in Drosophila and mammalian cells, revealing that this pathway is a critical gene regulatory mechanism.
Non-invasive mRNA stability measurements reveal that transcript lifetime is governed by a competition with translation initiation on a transcriptome-wide level.
To protect the germ line genome, chickens acquire new small RNA-based immune defense in response to recently endogenized retroviruses by turning a truncated provirus into a weapon.
Human plasma contains protein-protected mRNA fragments, myriad repeat RNAs, and novel intron RNAs, including a family of structured full-length excised introns, some corresponding to mirtron pre-miRNAs and agotrons.
