2,738 results found
    1. Neuroscience

    Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope

    Karen J Thompson et al.
    A new method allows silver- and gold-labeled tissues to be imaged with confocal microscopy, avoiding the problem of fluorescence photobleaching.
    1. Cell Biology
    2. Neuroscience

    Reconstruction of genetically identified neurons imaged by serial-section electron microscopy

    Maximilian Joesch et al.
    A new pipeline of electron microscopy techniques reduces the time required to visualize genetically targeted neurons and their connections by two orders of magnitude.
    1. Developmental Biology and Stem Cells
    2. Plant Biology

    Live tracking of moving samples in confocal microscopy for vertically grown roots

    Daniel von Wangenheim et al.
    A set of tools to image and track growing plant roots in optimized growing conditions within a vertical stage confocal microscope will open up new possibilities in research of root development.
    1. Neuroscience
    2. Developmental Biology and Stem Cells

    A novel optical microscope for imaging large embryos and tissue volumes with sub-cellular resolution throughout

    Gail McConnell et al.
    A new type of microscope reveals detail of organelles inside every cell in the entirety of an intact embryo or tissue region over 100 cubic millimeters in volume.
    1. Structural Biology and Molecular Biophysics

    Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

    Matteo Allegretti et al.
    The structure of a large enzyme complex involved in methanogenesis was determined at high resolution by electron cryo-microscopy.
    1. Structural Biology and Molecular Biophysics

    De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy

    Deryck J Mills et al.
    The structure of a large hydrogenase complex involved in the production of methane by microbes has been determined with cryo-electron microscopy.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli

    Dipali G Sashital et al.
    Comprehensive structural and compositional characterization of the in vivo 30S ribosomal assembly landscape reveals parallel pathways for 3′-domain formation and a previously unknown role for the assembly factor RimP.
    1. Structural Biology and Molecular Biophysics

    2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

    Melody G Campbell et al.
    Water molecules and amino-acid side chain rotamers have been resolved in the cryoEM structure of T20S proteasome at 2.8 angstroms.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps

    Abhishek Singharoy et al.
    New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.

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