A new open-source computational toolbox for processing in vivo microendoscopic calcium imaging data performs signal demixing and denoising much more accurately than previously available methods, significantly improving the utility of this imaging modality.

The Mouse Action Recognition System is a computational pipeline for automated classification of social behaviors in freely interacting mice, accompanied by a graphical interface for analysis of multimodal neuroscience datasets.

Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

CaImAn is an open-software package that equips the neuroscience community with a set of turnkey, fast and scalable solutions to pre-processing problems arising in single cell calcium imaging data analysis.

Excitatory output neurons in dorsomedial prefrontal cortex display heterogeneously specialized coding properties that evolve during cue-reward learning and predict cue-driven reward seeking.

Glutamatergic projection neurons downstream of cholinergic cells in the basal forebrain potently affect feeding behavior by modulating downstream reward and aversion circuits.

Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.

Distinct dorsal periaqueductal gray ensembles encode threat approach and avoidance behavioral states.

Activity levels in anterior and posterior cholinergic basal forebrain neurons are associated with distinct aspects of global brain state, sensory salience, reward expectation, and aversive reinforcement learning.

Threat and context-responsive neurons in the ventromedial hypothalamus are reshaped by experience to drive social avoidance.