Sophie Debaenst, Tamara Jarayseh ... Andy Willaert
Crispant analysis in zebrafish serves as a powerful tool for the rapid functional screening of genes implicated in bone fragility disorders, like osteogenesis imperfecta and osteoporosis.
Hongchang Zhao, Stella R Hartono ... Jacqueline Barlow
Genetic analysis of senataxin and RNase H2-deficient mice reveals that genome stability during class switch recombination and antibody generation can be uncoupled, suggesting a role for R loop metabolism dysfunction in lymphomagenesis in patients without immune deficiency.
Phenotypic diversity and cell state transition (i.e., acquisition of a CD44+/CD24- cell state or exposure to TGF-beta) can spur intra-tumor genetic heterogeneity and contribute to acquired resistance.
A combination of genetic and biochemical approaches suggest that yeast Rev7 promotes DNA double-strand break repair via NHEJ and inhibits homologous recombination by blocking Mre11 nuclease and Rad50’s ATPase activities.
To maintain genomic integrity during oocyte development, the tumor suppressor BRCA1/BRC-1 and the SMC-5/6 complex both repress intersister crossover recombination events while BRCA1/BRC-1 also specifically inhibits error prone repair of DNA breaks during meiotic prophase I.
Sumedha Dahal, Humaira Siddiqua ... Sathees C Raghavan
A new role for Endonuclease G with respect to the generation of mtDNA deletions is identified, which is dependent on the formation of G4 DNA within human mitochondria.
Chemically modifying the 5´ends of donor DNA improves the efficiency of homology-directed repair and suppresses end-joining reactions, which will facilitate precise gene editing.
